| Memphis & The Mississippi Delta | |||
| 1-1 | –Frank Stokes | 'Tain't Nobody's Business If I Do – Part II | 3:15 |
| 1-2 | –Cannon's Jug Stompers | Walk Right In | 2:57 |
| 1-3 | –Jim Jackson | Old Dog Blue | 3:04 |
| 1-4 | –Memphis Minnie & Kansas Joe | Pickin' The Blues | 3:05 |
| 1-5 | –Memphis Jug Band | Stealin' Stealin' | 3:11 |
| 1-6 | –Furry Lewis | Big Chief Blues | 2:53 |
| 1-7 | –Mississippi John Hurt | Avalon Blues | 3:05 |
| 1-8 | –Skip James | Cypress Grove Blues | 3:13 |
| 1-9 | –Charley Patton | High Sheriff Blues | 3:11 |
| 1-10 | –Son House | My Black Mama – Part II | 3:19 |
| 1-11 | –Robert Johnson | Crossroads Blues | 2:33 |
| 1-12 | –Big Joe Williams & Sonny Boy Williamson | Break 'Em On Down | 3:15 |
| 1-13 | –Bo Carter | Banana In Your Fruit Basket | 3:10 |
| 1-14 | –Mississippi Sheiks | Sitting On Top Of The World | 3:06 |
| 1-15 | –Tommy Johnson | Big Road Blues | 3:23 |
| 1-16 | –Muddy Waters | Country Blues (No. 1) | 3:24 |
| Alabama & The Eastern States | |||
| 2-1 | –Ed Bell (Barefoot Bill)* | Bad Boy | 3:05 |
| 2-2 | –Jaybird Coleman | Man Trouble Blues | 3:05 |
| 2-3 | –Lucille Bogan & Walter Roland | Down In Boogie Alley | 2:54 |
| 2-4 | –Papa Charlie Jackson | Papa's Lawdy Lawdy Blues | 2:36 |
| 2-5 | –Blind Blake | Hey Hey Daddy Blues | 3:13 |
| 2-6 | –Ma Rainey With Tampa Red And Georgia Tom | Black Eye Peas | 3:16 |
| 2-7 | –Peg Leg Howell | Coal Man Blues | 3:07 |
| 2-8 | –Georgia Cotton Pickers (Buddy Moss, Curley Weaver, Barbecue Bob)* | Diddle - Da - Diddle | 3:01 |
| 2-9 | –Andrew* & Jim Baxter | Georgia Stomp | 2:46 |
| 2-10 | –Blind Willie McTell | Love Changing Blues | 3:11 |
| 2-11 | –Josh White | Silicosis Is Killin' Me | 3:02 |
| 2-12 | –Blind Gary Davis | Cross And Evil Woman Blues | 3:05 |
| 2-13 | –Blind Boy Fuller & Sonny Terry | You Got To Have Your Dollar | 2:44 |
| 2-14 | –Luke Jordan | Cocaine Blues | 3:22 |
| South & The Mid-Western States | |||
| Texas | |||
| 3-1 | –Henry Thomas | Old Country Stomp | 2:54 |
| 3-2 | –Blind Lemon Jefferson | Matchbox Blues | 2:56 |
| 3-3 | –Blind Willie Johnson | God Moves On The Water | 3:02 |
| 3-4 | –Hattie Hudson | Doggone My Good Luck Soul | 3:10 |
| 3-5 | –Texas Alexander & Willie Reed | Easy Rider Blues | 2:48 |
| 3-6 | –J. T. "Funny Paper" Smith | Howling Wolf Blues – No. 1 | 2:52 |
| 3-7 | –Leadbelly | Four Day Worry Blues | 3:07 |
| Arkansas | |||
| 3-8 | –Buddy Boy Hawkins | A Rag | 2:57 |
| Louisiana | |||
| 3-9 | –King Solomon Hill | Down On My Bended Knee (Tk. 2) | 2:57 |
| 3-10 | –Amédé Ardoin | Blues De Basile | 3:05 |
| 3-11 | –Oscar Woods | Don't Sell It – Don't Give It Away | 2:34 |
| Kansas | |||
| 3-12 | –Lottie Beaman | Going Away Blues | 2:36 |
| Missouri | |||
| 3-13 | –Henry Townsend | Henry's Worry Blues | 3:09 |
| 3-14 | –Lonnie Johnson | Woke Up With The Blues In My Fingers | 3:12 |
| 3-15 | –Mary Johnson, Henry Brown, Ike Rodgers | Room Rent Blues | 2:48 |
| 3-16 | –Charley Jordan | Keep It Clean | 2:47 |
| 3-17 | –Roosevelt Sykes | Poor Boy Blues | 3:01 |
| 3-18 | –Sparks Brothers | "4-11-44" | 3:08 |